RNAi
RNA interference (RNAi) is the introduction of homologous double stranded RNA (dsRNA) to specifically target a gene's product resulting in null or hypomorphic phenotypes. [1] Phenotype results for NTRK1 mutations have only been identified in mice.
Model Organism Phenotype
In mice, homozygous null mutations result in premature death due to severe sensory and sympathetic neuropathies. A conditional mutant mouse exhibits defects in mast cell and B cell physiology. Homozygotes for a point mutation are normal, but are subject to pharmacological control of signalling. [2]
Chemical Genetics
PubChem was used to identify small molecules that interact with NTRK1. No small molecules of significance were identified using this method.
Micro Array
Gene Expression Omnibus (GEO) provides information about experiments and curated gene expression profiles. Gene expression levels of NTRK1 in patients with diabetic nephropathy were significantly higher than in normal patients. [3]
References
[1] "RNAi." RNAi. MAcalester College, n.d. Web. 15 May 2013.
[2] "Ntrk1 MGI Mouse Gene Detail - MGI:97383 - Neurotrophic Tyrosine Kinase, Receptor, Type 1." Ntrk1 MGI Mouse Gene Detail - MGI:97383 - Neurotrophic Tyrosine Kinase, Receptor, Type 1. Mouse Genome Database, 1 May 2013. Web. 15 May 2013.
[3] "NTRK1 - Diabetic Nephropathy." National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 15 May 2013.
[2] "Ntrk1 MGI Mouse Gene Detail - MGI:97383 - Neurotrophic Tyrosine Kinase, Receptor, Type 1." Ntrk1 MGI Mouse Gene Detail - MGI:97383 - Neurotrophic Tyrosine Kinase, Receptor, Type 1. Mouse Genome Database, 1 May 2013. Web. 15 May 2013.
[3] "NTRK1 - Diabetic Nephropathy." National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 15 May 2013.